45 research outputs found
Cell decision-making through the lens of Bayesian learning
Cell decision-making refers to the process by which cells gather information
from their local microenvironment and regulate their internal states to create
appropriate responses. Microenvironmental cell sensing plays a key role in this
process. Our hypothesis is that cell decision-making regulation is dictated by
Bayesian learning. In this article, we explore the implications of this
hypothesis for internal state temporal evolution. By using a timescale
separation between internal and external variables on the mesoscopic scale, we
derive a hierarchical Fokker-Planck equation for cell-microenvironment
dynamics. By combining this with the Bayesian learning hypothesis, we find that
changes in microenvironmental entropy dominate cell state probability
distribution. Finally, we use these ideas to understand how cell sensing
impacts cell decision-making. Notably, our formalism allows us to understand
cell state dynamics even without exact biochemical information about cell
sensing processes by considering a few key parameters
Avian photoreceptor patterns represent a disordered hyperuniform solution to a multiscale packing problem
Optimal spatial sampling of light rigorously requires that identical
photoreceptors be arranged in perfectly regular arrays in two dimensions.
Examples of such perfect arrays in nature include the compound eyes of insects
and the nearly crystalline photoreceptor patterns of some fish and reptiles.
Birds are highly visual animals with five different cone photoreceptor
subtypes, yet their photoreceptor patterns are not perfectly regular. By
analyzing the chicken cone photoreceptor system consisting of five different
cell types using a variety of sensitive microstructural descriptors, we find
that the disordered photoreceptor patterns are ``hyperuniform'' (exhibiting
vanishing infinite-wavelength density fluctuations), a property that had
heretofore been identified in a unique subset of physical systems, but had
never been observed in any living organism. Remarkably, the photoreceptor
patterns of both the total population and the individual cell types are
simultaneously hyperuniform. We term such patterns ``multi-hyperuniform''
because multiple distinct subsets of the overall point pattern are themselves
hyperuniform. We have devised a unique multiscale cell packing model in two
dimensions that suggests that photoreceptor types interact with both short- and
long-ranged repulsive forces and that the resultant competition between the
types gives rise to the aforementioned singular spatial features characterizing
the system, including multi-hyperuniformity.Comment: 31 pages, 12 figure
A Novel Averaging Principle Provides Insights in the Impact of Intratumoral Heterogeneity on Tumor Progression
From MDPI via Jisc Publications RouterHistory: accepted 2021-09-14, pub-electronic 2021-10-09Publication status: PublishedFunder: Mic2Mode-I2T; Grant(s): 01ZX1710B, 01ZX1308D, 01ZX1707C, 031L0085B, ZT-I- 392 0010, 96 732Typically stochastic differential equations (SDEs) involve an additive or multiplicative noise term. Here, we are interested in stochastic differential equations for which the white noise is nonlinearly integrated into the corresponding evolution term, typically termed as random ordinary differential equations (RODEs). The classical averaging methods fail to treat such RODEs. Therefore, we introduce a novel averaging method appropriate to be applied to a specific class of RODEs. To exemplify the importance of our method, we apply it to an important biomedical problem, in particular, we implement the method to the assessment of intratumoral heterogeneity impact on tumor dynamics. Precisely, we model gliomas according to a well-known Go or Grow (GoG) model, and tumor heterogeneity is modeled as a stochastic process. It has been shown that the corresponding deterministic GoG model exhibits an emerging Allee effect (bistability). In contrast, we analytically and computationally show that the introduction of white noise, as a model of intratumoral heterogeneity, leads to monostable tumor growth. This monostability behavior is also derived even when spatial cell diffusion is taken into account
In-silico insights on the prognostic potential of immune cell infiltration patterns in the breast lobular epithelium
Scattered inflammatory cells are commonly observed in mammary gland tissue, most likely in response to normal cell turnover by proliferation and apoptosis, or as part of immunosurveillance. In contrast, lymphocytic lobulitis (LLO) is a recurrent inflammation pattern, characterized by lymphoid cells infiltrating lobular structures, that has been associated with increased familial breast cancer risk and immune responses to clinically manifest cancer. The mechanisms and pathogenic implications related to the inflammatory microenvironment in breast tissue are still poorly understood. Currently, the definition of inflammation is mainly descriptive, not allowing a clear distinction of LLO from physiological immunological responses and its role in oncogenesis remains unclear. To gain insights into the prognostic potential of inflammation, we developed an agent-based model of immune and epithelial cell interactions in breast lobular epithelium. Physiological parameters were calibrated from breast tissue samples of women who underwent reduction mammoplasty due to orthopedic or cosmetic reasons. The model allowed to investigate the impact of menstrual cycle length and hormone status on inflammatory responses to cell turnover in the breast tissue. Our findings suggested that the immunological context, defined by the immune cell density, functional orientation and spatial distribution, contains prognostic information previously not captured by conventional diagnostic approaches. Several studies provided conclusive evidence that a delicate balance between mammary epithelial cell proliferation and apoptosis regulates homeostasis in the healthy breast tissue 1-7. After menarche, and in the absence of pregnancy, the adult female mammary gland is subjected to cyclic fluctuations depending on hormonal stimulation 1,8. In response to such systemic hormonal changes, the breast epithelium undergoes a tightly regulated sequence of cell proliferation and apoptosis during each ovarian/menstrual cycle 1-3. The peak of epithelial cell proliferation has been reported to occur during the luteal phase, suggesting a synergistic influence of steroid hormones, such as estrogen and progesterone 2-5. In turn, the peak of apoptotic activity would be expected in response to decreasing hormone levels towards the end of the menstrual cycle 2-5. However, recent histologic findings indicate that apoptosis reaches its maximum levels in the middle of the luteal phase, although there is also a peak at about the third day of the menstrual cycle 6,7. Experimental measurements of cell turnover, i.e. programmed cell death and proliferation, demonstrated that an imbalance between the mitotic and apoptotic activity might lead to malignant transformation of epithelial cells and tumorigenic processes 9-11. Indeed, excessive cell proliferation promotes accumulation of DNA damage due to insufficient timely repair and mutations 12,13. There is also recent evidence that hormones suppress effective DNA repair and alter DNA damage response (DDR) 13-15
Hook length of the bacterial flagellum is optimized for maximal stability of the flagellar bundle
Most bacteria swim in liquid environments by rotating one or several flagella. The long external filament of the flagellum is connected to a membrane-embedded basal body by a flexible universal joint, the hook, which allows the transmission of motor torque to the filament. The length of the hook is controlled on a nanometer scale by a sophisticated molecular ruler mechanism. However, why its length is stringently controlled has remained elusive. We engineered and studied a diverse set of hook- length variants of Salmonella enterica. Measurements of plate-assay motility, single- cell swimming speed, and directional persistence in quasi-2D and population- averaged swimming speed and body angular velocity in 3D revealed that the motility performance is optimal around the wild-type hook length. We conclude that too-short hooks may be too stiff to function as a junction and too-long hooks may buckle and create instability in the flagellar bundle. Accordingly, peritrichously flagellated bacteria move most efficiently as the distance travelled per body rotation is maximal and body wobbling is minimized. Thus, our results suggest that the molecular ruler mechanism evolved to control flagellar hook growth to the optimal length consistent with efficient bundle formation. The hook-length control mechanism is therefore a prime example of how bacteria evolved elegant but robust mechanisms to maximize their fitness under specific environmental constraints
Decreased plasma phospholipid concentrations and increased acid sphingomyelinase activity are accurate biomarkers for community-acquired pneumonia
Background: There continues to be a great need for better biomarkers and host-directed treatment targets for
community-acquired pneumonia (CAP). Alterations in phospholipid metabolism may constitute a source of small
molecule biomarkers for acute infections including CAP. Evidence from animal models of pulmonary infections and
sepsis suggests that inhibiting acid sphingomyelinase (which releases ceramides from sphingomyelins) may reduce
end-organ damage.
Methods: We measured concentrations of 105 phospholipids, 40 acylcarnitines, and 4 ceramides, as well as acid
sphingomyelinase activity, in plasma from patients with CAP (n=29, sampled on admission and 4 subsequent time
points), chronic obstructive pulmonary disease exacerbation with infection (COPD, n=13) as a clinically important
disease control, and 33 age- and sex-matched controls.
Results: Phospholipid concentrations were greatly decreased in CAP and normalized along clinical improvement.
Greatest changes were seen in phosphatidylcholines, followed by lysophosphatidylcholines, sphingomyelins and cerâ
amides (three of which were upregulated), and were least in acylcarnitines. Changes in COPD were less pronounced,
but also difered qualitatively, e.g. by increases in selected sphingomyelins. We identifed highly accurate biomarkâ
ers for CAP (AUCâ€0.97) and COPD (AUCâ€0.93) vs. Controls, and moderately accurate biomarkers for CAP vs. COPD
(AUCâ€0.83), all of which were phospholipids. Phosphatidylcholines, lysophosphatidylcholines, and sphingomyelins
were also markedly decreased in S. aureus-infected human A549 and diferentiated THP1 cells. Correlations with
C-reactive protein and procalcitonin were predominantly negative but only of mild-to-moderate extent, suggesting
that these markers refect more than merely infammation. Consistent with the increased ceramide concentrations,
increased acid sphingomyelinase activity accurately distinguished CAP (fold change=2.8, AUC=0.94) and COPD
(1.75, 0.88) from Controls and normalized with clinical resolution Conclusions: The results underscore the high potential of plasma phospholipids as biomarkers for CAP, begin to
reveal diferences in lipid dysregulation between CAP and infection-associated COPD exacerbation, and suggest that
the decreases in plasma concentrations are at least partially determined by changes in host target cells. Furthermore,
they provide validation in clinical blood samples of acid sphingomyelinase as a potential treatment target to improve
clinical outcome of CAP
A Novel Averaging Principle Provides Insights in the Impact of Intratumoral Heterogeneity on Tumor Progression
Typically stochastic differential equations (SDEs) involve an additive or multiplicative noise term. Here, we are interested in stochastic differential equations for which the white noise is nonlinearly integrated into the corresponding evolution term, typically termed as random ordinary differential equations (RODEs). The classical averaging methods fail to treat such RODEs. Therefore, we introduce a novel averaging method appropriate to be applied to a specific class of RODEs. To exemplify the importance of our method, we apply it to an important biomedical problem, in particular, we implement the method to the assessment of intratumoral heterogeneity impact on tumor dynamics. Precisely, we model gliomas according to a well-known Go or Grow (GoG) model, and tumor heterogeneity is modeled as a stochastic process. It has been shown that the corresponding deterministic GoG model exhibits an emerging Allee effect (bistability). In contrast, we analytically and computationally show that the introduction of white noise, as a model of intratumoral heterogeneity, leads to monostable tumor growth. This monostability behavior is also derived even when spatial cell diffusion is taken into account